6zvy | pdb_00006zvy

X-RAY STRUCTURE OF THE HALOALKANE DEHALOGENASE HALOTAG7-Q165H-P174R LABELED WITH A CHLOROALKANE-TETRAMETHYLRHODAMINE FLUOROPHORE SUBSTRATE

spinqualitylabelsall models 6zvy, resolution 1.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6zvy is a 2 chain structure with sequence from Rhoso. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CL, GOL, OEH Related: 6zvu, 6zvv, 6zvx, 6zvw Gene: dhaA (RHOSO) Activity: Haloalkane dehalogenase, with EC number 3.8.1.5 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Self-labeling protein tags such as HaloTag are powerful tools that can label fusion proteins with synthetic fluorophores for use in fluorescence microscopy. Here we introduce HaloTag variants with either increased or decreased brightness and fluorescence lifetime compared with HaloTag7 when labeled with rhodamines. Combining these HaloTag variants enabled live-cell fluorescence lifetime multiplexing of three cellular targets in one spectral channel using a single fluorophore and the generation of a fluorescence lifetime-based biosensor. Additionally, the brightest HaloTag variant showed up to 40% higher brightness in live-cell imaging applications. Engineered HaloTag variants for fluorescence lifetime multiplexing.,Frei MS, Tarnawski M, Roberti MJ, Koch B, Hiblot J, Johnsson K Nat Methods. 2022 Jan;19(1):65-70. doi: 10.1038/s41592-021-01341-x. Epub 2021 Dec, 16. PMID:34916672 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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