7ohf | pdb_00007ohf

Cryo-EM structure of pyrococcus furiosus apoferritin in nanofluidic channels

spinqualitylabelsall models 7ohf, resolution 3.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7ohf is a 1 chain structure with sequence from Pyrococcus furiosus com1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: PFC_02870 (Pyrococcus furiosus COM1) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Cryogenic electron microscopy has become an essential tool for structure determination of biological macromolecules. In practice, the difficulty to reliably prepare samples with uniform ice thickness still represents a barrier for routine high-resolution imaging and limits the current throughput of the technique. We show that a nanofluidic sample support with well-defined geometry can be used to prepare cryo-EM specimens with reproducible ice thickness from picoliter sample volumes. The sample solution is contained in electron-transparent nanochannels that provide uniform thickness gradients without further optimisation and eliminate the potentially destructive air-water interface. We demonstrate the possibility to perform high-resolution structure determination with three standard protein specimens. Nanofabricated sample supports bear potential to automate the cryo-EM workflow, and to explore new frontiers for cryo-EM applications such as time-resolved imaging and high-throughput screening. Nanofluidic chips for cryo-EM structure determination from picoliter sample volumes.,Huber ST, Sarajlic E, Huijink R, Weis F, Evers WH, Jakobi AJ Elife. 2022 Jan 21;11. pii: 72629. doi: 10.7554/eLife.72629. PMID:35060902 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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