3fjg | pdb_00003fjg

Crystal structure of 3PG bound PEB3

spinqualitylabelsall models 3fjg, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3fjg is a 4 chain structure with sequence from "campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp. jejuni" smibert 1974. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 3PG Related: 2hxw, 3fir, 3fj7, 3fjm Gene: Cj0289c, peb3 ("Campylobacter fetus subsp. jejuni" Smibert 1974) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed PEB3 is a glycoprotein adhesin from C. jejuni whose structure suggested a role in transport. We have investigated potential ligands for PEB3 and characterized their binding properties using biophysical methods in solution and by X-ray crystallography. A thermal aggregation assay of PEB3 with a library of physiological compounds identified three possible ligands (3-phosphoglycerate (3-PG), phosphoenolpyruvate (PEP) and aconitate), which stabilized wild type PEB3 but did not stabilize either a PEB3 form containing two mutations at the ligand-binding site, T138A/S139A, or a second PEB3 mutant, K135E, at a site ~14 A away. Fluorescence titration experiments and co-crystal structures with various ligands were used to characterize the binding of 3-PG, PEP and phosphate to PEB3. Further, a C. jejuni growth experiment in minimal medium supplemented with 3-PG showed that this molecule enhances the growth of wild type C. jejuni, but not of the PEB3 mutants. Crystallographic analysis of PEB3 complexes revealed that the region Ser171-Gln180, in the presence of 3-PG, or other phosphates, is helical and similar to other transport proteins, but it is non-helical when citrate is bound. The K135E mutation resulted in expression of a more highly glycosylated form of PEB3 in vivo and its crystal structure showed the conformation of the first two residues of the glycan. Based on our findings, we suggest that PEB3 is a transport protein that may function in utilization of 3-PG or other phosphate-containing molecules from the host. Specificity of the Campylobacter jejuni Adhesin PEB3 for Phosphates and Structural Differences among its Ligand Complexes.,Min T, Vedadi M, Watson D, Wasney G, Munger C, Cygler M, Matte A, Young N Biochemistry. 2009 Feb 23. PMID:19236052 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA