7nhf | pdb_00007nhf

Crystal structure of Arabidopsis thaliana Pdx1K166R

spinqualitylabelsall models 7nhf, resolution 2.35Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7nhf is a 4 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: PO4 Activity: Pyridoxal 5'-phosphate synthase (glutamine hydrolyzing), with EC number 4.3.3.6 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [PDX13_ARATH] Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by PDX2. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively. Also plays an indirect role in resistance to singlet oxygen-generating photosensitizers. Publication Abstract from PubMed The Pdx1 enzyme catalyses condensation of two carbohydrates and ammonia to form pyridoxal 5-phosphate (PLP) via an imine relay mechanism of carbonyl intermediates. The I333 intermediate characterised here using structural, UV-vis absorption spectroscopy and mass spectrometry analyses rationalises stereoselective deprotonation and subsequent substrate assisted phosphate elimination, central to PLP biosynthesis. Trapping and structural characterisation of a covalent intermediate in vitamin B6 biosynthesis catalysed by the Pdx1 PLP synthase.,Rodrigues MJ, Giri N, Royant A, Zhang Y, Bolton R, Evans G, Ealick SE, Begley T, Tews I RSC Chem Biol. 2021 Oct 25;3(2):227-230. doi: 10.1039/d1cb00160d. eCollection, 2022 Feb 9. PMID:35360887 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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