2x2q | pdb_00002x2q

Crystal structure of an 'all locked' LNA duplex at 1.9 angstrom resolution

spinqualitylabelsall models 2x2q, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2x2q is a 4 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CAC, MG, NCO NonStd Res: LCA, LCC, LCG, TLN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed 'Locked nucleic acids' (LNAs) are known to introduce enhanced bio- and thermostability into natural nucleic acids rendering them powerful tools for diagnostic and therapeutic applications. We present the 1.9 A X-ray structure of an 'all LNA' duplex containing exclusively modified beta-D-2'-O-4'C-methylene ribofuranose nucleotides. The helix illustrates a new type of nucleic acid geometry that contributes to the understanding of the enhanced thermostability of LNA duplexes. A notable decrease of several local and overall helical parameters like twist, roll and propeller twist influence the structure of the LNA helix and result in a widening of the major groove, a decrease in helical winding and an enlarged helical pitch. A detailed structural comparison to the previously solved RNA crystal structure with the corresponding base pair sequence underlines the differences in conformation. The surrounding water network of the RNA and the LNA helix shows a similar hydration pattern. The crystal structure of an 'All Locked' nucleic acid duplex.,Eichert A, Behling K, Betzel C, Erdmann VA, Furste JP, Forster C Nucleic Acids Res. 2010 Oct 1;38(19):6729-36. Epub 2010 Jun 8. PMID:20530536 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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