2xdb | pdb_00002xdb

A processed non-coding RNA regulates a bacterial antiviral system

spinqualitylabelsall models 2xdb, resolution 2.55Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2xdb is a 2 chain structure with sequence from [1] and "bacillus_atrosepticus"_van_hall_1902 "bacillus atrosepticus" van hall 1902. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CO, SO4 NonStd Res: A23 Related: 2xd0, 2xdd Activity: Ribonuclease T(1), with EC number 3.1.27.3 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The >/=10(30) bacteriophages on Earth relentlessly drive adaptive coevolution, forcing the generation of protective mechanisms in their bacterial hosts. One such bacterial phage-resistance system, ToxIN, consists of a protein toxin (ToxN) that is inhibited in vivo by a specific RNA antitoxin (ToxI); however, the mechanisms for this toxicity and inhibition have not been defined. Here we present the crystal structure of the ToxN-ToxI complex from Pectobacterium atrosepticum, determined to 2.75-A resolution. ToxI is a 36-nucleotide noncoding RNA pseudoknot, and three ToxI monomers bind to three ToxN monomers to generate a trimeric ToxN-ToxI complex. Assembly of this complex is mediated entirely through extensive RNA-protein interactions. Furthermore, a 2'-3' cyclic phosphate at the 3' end of ToxI, and catalytic residues, identify ToxN as an endoRNase that processes ToxI from a repetitive precursor but is regulated by its own catalytic product. A processed noncoding RNA regulates an altruistic bacterial antiviral system.,Blower TR, Pei XY, Short FL, Fineran PC, Humphreys DP, Luisi BF, Salmond GP Nat Struct Mol Biol. 2011 Feb;18(2):185-90. Epub 2011 Jan 16. PMID:21240270 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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