BASIL2022GV3HDT
From Proteopedia
Characterizing Putative Kinase 3HDT
| ||||||||||||
References
Proteopedia Page Contributors and Editors (what is this?)
Autumn Forrester, Jesse D. Rothfus, Jaime Prilusky, Bonnie Hall
This is a default text for your page BASIL2022GV3HDT. Click above on edit this page to modify. Be careful with the < and > signs. You may include any references to papers as in: the use of JSmol in Proteopedia [1] or to the article describing Jmol [2] to the rescue. This protein (3HDT) is a putative kinase with possibly cytidylate kinase function utilizing ATP and dCMP as ligands. ContentsIntroductionOnline Resources (bioinformatic software)We used a variety of in silico databases with our protein, 3HDT to find similarities with other amino acid sequences, protein family matches, and structural comparisons to known proteins in the PDB. Below are the recorded results and information from each database. From this information, a hypothesized function was created for 3HDT and potential substrates were selected such as dCMP. BLASTpPfamDALIPANZDockingLaboratory ExperimentsCoupled kinase assaySDS-PAGESDS-PAGE results for the purified protein 3HDT. The total weight of this protein is around 25.79 kD. The first lane (left) contains a size standard. The band in the second lane (right) at ~70kD is not the protein of interest (3HDT) but contains a binding metal protein. There is also a faint band around ~26kD, indicating our protein of interest was present.
Conclusion/Future ExperimentsThis is a sample scene created with SAT to color by Group, and another to make a transparent representation of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.
| ||||||||||||
Autumn Forrester, Jesse D. Rothfus, Jaime Prilusky, Bonnie Hall
This page was last modified 01:12, 20 April 2022.