6ye4 | pdb_00006ye4

Structure of ExbB pentamer from Serratia marcescens by single particle cryo electron microscopy

spinqualitylabelsall models 6ye4, resolution 3.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6ye4 is a 5 chain structure with sequence from "bacillus_marcescens"_(bizio_1823)_trevisan_in_de_toni_and_trevisan_1889 "bacillus marcescens" (bizio 1823) trevisan in de toni and trevisan 1889. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: PGT Gene: exbB, FG174_21755, PWN146_03792 ("Bacillus marcescens" (Bizio 1823) Trevisan in de Toni and Trevisan 1889) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed ExbB and ExbD are cytoplasmic membrane proteins that associate with TonB to convey the energy of the proton-motive force to outer membrane receptors in Gram-negative bacteria for iron uptake. The opportunistic pathogen Serratia marcescens (Sm) possesses both TonB and a heme-specific TonB paralog, HasB. ExbBSm has a long periplasmic extension absent in other bacteria such as E. coli (Ec). Long ExbB's are found in several genera of Alphaproteobacteria, most often in correlation with a hasB gene. We investigated specificity determinants of ExbBSm and HasB. We determined the cryo-EM structures of ExbBSm and of the ExbB-ExbDSm complex from S. marcescens. ExbBSm alone is a stable pentamer, and its complex includes two ExbD monomers. We showed that ExbBSm extension interacts with HasB and is involved in heme acquisition and we identified key residues in the membrane domain of ExbBSm and ExbBEc, essential for function and likely involved in the interaction with TonB/HasB. Our results shed light on the class of inner membrane energy machinery formed by ExbB, ExbD and HasB. Structural and molecular determinants for the interaction of ExbB from Serratia marcescens and HasB, a TonB paralog.,Biou V, Adaixo RJD, Chami M, Coureux PD, Laurent B, Enguene VYN, de Amorim GC, Izadi-Pruneyre N, Malosse C, Chamot-Rooke J, Stahlberg H, Delepelaire P Commun Biol. 2022 Apr 13;5(1):355. doi: 10.1038/s42003-022-03306-y. PMID:35418619 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also ExbB

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