3ajn | pdb_00003ajn

Structural basis of glycine amide on suppression of protein aggregation by high resolution X-ray analysis

spinqualitylabelsall models 3ajn, resolution 1.05Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3ajn is a 1 chain structure with sequence from Gallus gallus. This structure supersedes the now removed PDB entry 2zxs. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CL, GM1, NA Activity: Lysozyme, with EC number 3.2.1.17 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus. Publication Abstract from PubMed Glycine amide (GlyAd), a typically amidated amino acid, is a versatile additive that suppresses protein aggregation during refolding, heat treatment, and crystallization. In spite of its effectiveness, the exact mechanism by which GlyAd suppresses protein aggregation remains to be elucidated. Here, we show the crystal structure of the GlyAd-lysozyme complex by high resolution X-ray crystallographic analysis at a 1.05A resolution. GlyAd bound to the lysozyme surface near aromatic residues and decreased the amount of bound waters and increased the mobility of protein. Arg and GlyAd molecules are different in binding sites and patterns from glycerol and related compounds, indicating that decreasing hydrophobic patches might be involved in suppression of protein aggregation. Glycine amide shielding on the aromatic surfaces of lysozyme: Implication for suppression of protein aggregation.,Ito L, Shiraki K, Makino M, Hasegawa K, Kumasaka T FEBS Lett. 2011 Feb 4;585(3):555-60. Epub 2011 Jan 14. PMID:21237160 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Lysozyme 3D structures

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