3ja4 | pdb_00003ja4

RNA-dependent RNA polymerases of transcribing cypoviruses

Loading... Status: waiting for Mol* initialization Structural highlights 3ja4 is a 1 chain structure with sequence from Bombyx mori cypovirus 1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 3ja5 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Double-stranded RNA (dsRNA) viruses possess a segmented dsRNA genome and a number of RNA-dependent RNA polymerases (RdRps) enclosed in a capsid. Until now, the precise structures of genomes and RdRps within the capsids have been unknown. Here we report the structures of RdRps and associated RNAs within nontranscribing and transcribing cypoviruses (NCPV and TCPV, respectively), using a combination of cryo-electron microscopy (cryo-EM) and a symmetry-mismatch reconstruction method. The RdRps and associated RNAs appear to exhibit a pseudo-D3 symmetric organization in both NCPV and TCPV. However, the molecular interactions between RdRps and the genomic RNA were found to differ in these states. Our work provides insight into the mechanisms of the replication and transcription in dsRNA viruses and paves a way for structural determination of lower-symmetry complexes enclosed in higher-symmetry structures. Cryo-EM shows the polymerase structures and a nonspooled genome within a dsRNA virus.,Liu H, Cheng L Science. 2015 Sep 18;349(6254):1347-50. doi: 10.1126/science.aaa4938. PMID:26383954 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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