3o27 | pdb_00003o27

The crystal structure of C68 from the hybrid virus-plasmid pSSVx

spinqualitylabelsall models 3o27, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3o27 is a 2 chain structure with sequence from "sulfolobus_islandicus"_zillig_et_al._1994 "sulfolobus islandicus" zillig et al. 1994. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: orfc68 ("Sulfolobus islandicus" Zillig et al. 1994) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The genetic element pSSVx from Sulfolobus islandicus, strain REY15/4, is a hybrid between a plasmid and a fusellovirus. This plasmid/virus hybrid infects several species of the hyperthermophilic, acidophilic crenarchaeon Sulfolobus. The open reading frame c68 of pSSVx encodes for a 7.7 kDa protein that does not show significant sequence homology to any protein with known 3D structure. EMSA experiments, DNA footprinting and CD analyses indicate that recombinant C68, purified from Escherichia coli, binds to two different operator sites, that are located upstream of its own promoter. The three-dimensional structure, solved by a single wavelength anomalous diffraction experiment on a selenomethionine derivative, shows that the protein assumes a swapped-hairpin fold, which is a distinctive fold associated with a family of prokaryotic transcription factors, such as AbrB from Bacillus subtilis. Nevertheless, C68 constitutes a novel representative of this family because it shows several peculiar structural and functional features. c68 from the Sulfolobus islandicus plasmid/virus pSSVx is a novel member of the AbrB-like transcription factor family.,Contursi P, D'Ambrosio K, Pirone L, Pedone E, Aucelli T, She Q, De Simone G, Bartolucci S Biochem J. 2011 Jan 5. PMID:21208189 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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