7ek2 | pdb_00007ek2

Cryo-EM structure of VCCN1 in lipid nanodisc

spinqualitylabelsall models 7ek2, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7ek2 is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed In the light reaction of plant photosynthesis, modulation of electron transport chain reactions is important to maintain the efficiency of photosynthesis under a broad range of light intensities. VCCN1 was recently identified as a voltage-gated chloride channel residing in the thylakoid membrane, where it plays a key role in photoreaction tuning to avoid the generation of reactive oxygen species (ROS). Here, we present the cryo-EM structures of Malus domestica VCCN1 (MdVCCN1) in nanodiscs and detergent at 2.7 A and 3.0 A resolutions, respectively, and the structure-based electrophysiological analyses. VCCN1 structurally resembles its animal homolog, bestrophin, a Ca(2+)-gated anion channel. However, unlike bestrophin channels, VCCN1 lacks the Ca(2+)-binding motif but instead contains an N-terminal charged helix that is anchored to the lipid membrane through an additional amphipathic helix. Electrophysiological experiments demonstrate that these structural elements are essential for the channel activity, thus revealing the distinct activation mechanism of VCCN1. Cryo-EM structures of thylakoid-located voltage-dependent chloride channel VCCN1.,Hagino T, Kato T, Kasuya G, Kobayashi K, Kusakizako T, Hamamoto S, Sobajima T, Fujiwara Y, Yamashita K, Kawasaki H, Maturana AD, Nishizawa T, Nureki O Nat Commun. 2022 May 6;13(1):2505. doi: 10.1038/s41467-022-30292-w. PMID:35523970 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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