3om2 | pdb_00003om2

Crystal structure of B. megaterium levansucrase mutant D257A

spinqualitylabelsall models 3om2, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3om2 is a 1 chain structure with sequence from Atcc 14581. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CA, CIT, MG Related: 3om4, 3om5, 3om6, 3om7 Gene: sacB (ATCC 14581) Activity: Levansucrase, with EC number 2.4.1.10 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Despite the widespread biological function of carbohydrates, the polysaccharide synthesis mechanisms of glycosyltransferases remain largely unexplored. Bacterial levansucrases (glycoside hydrolase family 68) synthesize high molecular weight, beta-(2,6)-linked levan from sucrose by transfer of fructosyl units. The kinetic and biochemical characterization of Bacillus megaterium levansucrase SacB variants Y247A, Y247W, N252A, D257A, and K373A reveal novel surface motifs remote from the sucrose binding site with distinct influence on the polysaccharide product spectrum. The wild type activity (k(cat)) and substrate affinity (K(m)) are maintained. The structures of the SacB variants reveal clearly distinguishable subsites for polysaccharide synthesis as well as an intact active site architecture. These results lead to a new understanding of polysaccharide synthesis mechanisms. The identified surface motifs are discussed in the context of related glycosyltransferases. Polysaccharide Synthesis of the Levansucrase SacB from Bacillus megaterium Is Controlled by Distinct Surface Motifs.,Strube CP, Homann A, Gamer M, Jahn D, Seibel J, Heinz DW J Biol Chem. 2011 May 20;286(20):17593-600. Epub 2011 Mar 25. PMID:21454585 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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