3pyw | pdb_00003pyw

The structure of the SLH domain from B. anthracis surface array protein at 1.8A

spinqualitylabelsall models 3pyw, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3pyw is a 1 chain structure with sequence from "bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var. anthracis" (cohn 1872) smith et al. 1946. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: SO4 Gene: BAS0841, BA_0885, GBAA_0885, GI:49183865, sap ("Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [SLAP1_BACAN] The S-layer is a paracrystalline mono-layered assembly of proteins which coat the surface of bacteria. Publication Abstract from PubMed Surface (S)-layers, para-crystalline arrays of protein, are deposited in the envelope of most bacterial species. These surface organelles are retained in the bacterial envelope through the non-covalent association of proteins with cell wall carbohydrates. Bacillus anthracis, a Gram-positive pathogen, produces S-layers of the protein Sap, which uses three consecutive repeats of the surface-layer homology (SLH) domain to engage secondary cell wall polysaccharides (SCWP). Using x-ray crystallography, we reveal here the structure of these SLH domains, which assume the shape of a three-prong spindle. Each SLH domain contributes to a three-helical bundle at the spindle base, whereas another alpha-helix and its connecting loops generate the three prongs. The inter-prong grooves contain conserved cationic and anionic residues, which are necessary for SLH domains to bind the B. anthracis SCWP. Modeling experiments suggest that the SLH domains of other S-layer proteins also fold into three-prong spindles and capture bacterial envelope carbohydrates by a similar mechanism. Structure of Surface Layer Homology (SLH) Domains from Bacillus anthracis Surface Array Protein.,Kern J, Wilton R, Zhang R, Binkowski TA, Joachimiak A, Schneewind O J Biol Chem. 2011 Jul 22;286(29):26042-9. Epub 2011 May 13. PMID:21572039 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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