3qr7 | pdb_00003qr7

Crystal structure of the C-terminal fragment of the bacteriophage P2 membrane-piercing protein gpV

spinqualitylabelsall models 3qr7, resolution 0.94Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3qr7 is a 2 chain structure with sequence from Bpp2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CA, CL, FE, NA Related: 3qr8 Gene: V (BPP2) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [VPV_BPP2] Forms the small spike at the tip of the tail. Publication Abstract from PubMed Bacteriophages with contractile tails and the bacterial type VI secretion system have been proposed to use a special protein to create an opening in the host cell membrane during infection. These proteins have a modular architecture but invariably contain an oligonucleotide/oligosaccharide-binding (OB-fold) domain and a long beta-helical C-terminal domain, which initiates the contact with the host cell membrane. Using X-ray crystallography and electron microscopy, we report the atomic structure of the membrane-piercing proteins from bacteriophages P2 and varphi92 and identify the residues that constitute the membrane-attacking apex. Both proteins form compact spikes with a approximately 10A diameter tip that is stabilized by a centrally positioned iron ion bound by six histidine residues. The accumulated data strongly suggest that, in the process of membrane penetration, the spikes are translocated through the lipid bilayer without undergoing major unfolding. Phage pierces the host cell membrane with the iron-loaded spike.,Browning C, Shneider MM, Bowman VD, Schwarzer D, Leiman PG Structure. 2012 Feb 8;20(2):326-39. PMID:22325780 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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