3gx5 | pdb_00003gx5

Crystal structure of T. tencongensis SAM-I riboswitch variant A94G/U34 bound with SAM

spinqualitylabelsall models 3gx5, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3gx5 is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: K, MG, SAM Related: 3gx2, 3gx3, 3gx6, 3gx7 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The SAM-I riboswitch is a cis-acting element of genetic control found in bacterial mRNAs that specifically binds S-adenosylmethionine (SAM). We previously determined the 2.9-A X-ray crystal structure of the effector-binding domain of this RNA element, revealing details of RNA-ligand recognition. To improve this structure, variations were made to the RNA sequence to alter lattice contacts, resulting in a 0.5-A improvement in crystallographic resolution and allowing for a more accurate refinement of the crystallographic model. The basis for SAM specificity was addressed by a structural analysis of the RNA complexed to S-adenosylhomocysteine (SAH) and sinefungin and by measuring the affinity of SAM and SAH for a series of mutants using isothermal titration calorimetry. These data illustrate the importance of two universally conserved base pairs in the RNA that form electrostatic interactions with the positively charged sulfonium group of SAM, thereby providing a basis for discrimination between SAM and SAH. Discrimination between closely related cellular metabolites by the SAM-I riboswitch.,Montange RK, Mondragon E, van Tyne D, Garst AD, Ceres P, Batey RT J Mol Biol. 2010 Feb 26;396(3):761-72. Epub 2009 Dec 16. PMID:20006621 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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