3s5v | pdb_00003s5v

Structure of the cyanobacterial Oscillatoria Agardhii Agglutinin (OAA) in free state obtained at -180 degrees Celsius

spinqualitylabelsall models 3s5v, resolution 1.55Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3s5v is a 1 chain structure with sequence from "planktothrix_agardhii"_emend._suda_et_al._2002 "planktothrix agardhii" emend. suda et al. 2002. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: SO4 Related: 3obl, 3s5x, 3s60 Gene: OAA ("Planktothrix agardhii" emend. Suda et al. 2002) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The cyanobacterial Oscillatory Agardhii agglutinin (OAA) is a recently discovered HIV-inactivating lectin that interacts with high-mannose sugars. Nuclear magnetic resonance (NMR) binding studies between OAA and alpha3,alpha6-mannopentaose (Manalpha(1-3)[Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)]Man), the branched core unit of Man-9, revealed two binding sites at opposite ends of the protein, exhibiting essentially identical affinities. Atomic details of the specific protein-sugar contacts in the recognition loops of OAA were delineated in the high-resolution crystal structures of free and glycan-complexed protein. No major changes in the overall protein structure are induced by carbohydrate binding, with essentially identical apo- and sugar-bound conformations in binding site 1. A single peptide bond flip at W77-G78 is seen in binding site 2. Our combined NMR and crystallographic results provide structural insights into the mechanism by which OAA specifically recognizes the branched Man-9 core, distinctly different from the recognition of the D1 and D3 arms at the nonreducing end of high-mannose carbohydrates by other antiviral lectins. Structural basis of the anti-HIV activity of the cyanobacterial Oscillatoria Agardhii agglutinin.,Koharudin LM, Gronenborn AM Structure. 2011 Aug 10;19(8):1170-81. PMID:21827952 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Agglutinin 3D structures

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