3ifi | pdb_00003ifi

2'-SeMe-dG modified octamer DNA

spinqualitylabelsall models 3ifi, resolution 1.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3ifi is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. NonStd Res: XUG Related: 1dns, 3iff Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Selenium modification of nucleic acids is of great importance in X-ray crystal structure determination and functional study of nucleic acids. Herein, we describe a convenient synthesis of a new building block, the 2'-SeMe-modified guanosine (G(Se)) phosphoramidite, and report the first incorporation of the 2'-Se-G moiety into DNA. The X-ray crystal structure of the 2'-Se-modified octamer DNA (5'-GTG(Se)TACAC-3') was determined at a resolution of 1.20 A. We also found that the 2'-Se modification points to the minor groove and that the modified and native structures are virtually identical. Furthermore, we observed that the 2'-Se-G modification can significantly facilitate the crystal growth with respect to the corresponding native DNA. Synthesis and crystal structure of 2'-se-modified guanosine containing DNA.,Salon J, Sheng J, Gan J, Huang Z J Org Chem. 2010 Feb 5;75(3):637-41. PMID:20047333 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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