3so2 | pdb_00003so2

Chlorella dUTPase

spinqualitylabelsall models 3so2, resolution 1.64Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3so2 is a 1 chain structure with sequence from Chlva. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: CHLNCDRAFT_27939 (CHLVA) Activity: dUTP diphosphatase, with EC number 3.6.1.23 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Deoxyuridine triphosphatase (dUTPase) is a ubiquitous enzyme that has been widely studied owing to its function and evolutionary significance. The gene coding for the dUTPase from the Chlorella alga was codon-optimized and synthesized. The synthetic gene was expressed in Escherichia coli and recombinant core Chlorella dUTPase (chdUTPase) was purified. Crystallization of chdUTPase was performed by the repetitive hanging-drop vapor-diffusion method at 298 K with ammonium sulfate as the precipitant. In the presence of 2'-deoxyuridine-5'-[(alpha,beta)-imido]triphosphate and magnesium, the enzyme produced die-shaped hexagonal R3 crystals with unit-cell parameters a = b = 66.9, c = 93.6 A, gamma = 120 degrees . X-ray diffraction data for chdUTPase were collected to 1.6 A resolution. The crystallization of chdUTPase with manganese resulted in very fragile clusters of needles. Crystallization of Chlorella deoxyuridine triphosphatase.,Badalucco L, Poudel I, Yamanishi M, Natarajan C, Moriyama H Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Dec 1;67(Pt 12):1599-602. , Epub 2011 Nov 26. PMID:22139176 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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