3thk | pdb_00003thk

Structure of SH3 chimera with a type II ligand linked to the chain C-terminal

spinqualitylabelsall models 3thk, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3thk is a 4 chain structure with sequence from Buffalo rat. This structure supersedes the now removed PDB entry 2pqh. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: BME, SO4 Related: 1pwt, 1shg Gene: Sptan1, Spna2, Spta2 (Buffalo rat) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [SPTN1_RAT] Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. Publication Abstract from PubMed A new chimeric protein, named WT-CIIA, was designed by connecting the proline-rich decapeptide PPPVPPYSAG to the C-terminus of the alpha-spectrin SH3 domain through a natural twelve-residue linker to obtain a single-chain model that would imitate intramolecular SH3-ligand interaction. The crystal structure of this fusion protein was determined at 1.7 A resolution. The asymmetric unit of the crystal contained two SH3 globules contacting with one PPPVPPY fragment located between them. The domains are related by the two-fold non-crystallographic axis and the ligand lies in two opposite orientations with respect to the conservative binding sites of SH3 domains. High-Resolution Crystal Structure of Spectrin SH3 Domain Fused with a Proline-Rich Peptide.,Gushchina LV, Gabdulkhakov AG, Nikonov SV, Filimonov VV J Biomol Struct Dyn. 2011 Dec;29(3):485-95. PMID:22066535 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Spectrin 3D structures

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