3v6k | pdb_00003v6k

Replication of N2,3-Ethenoguanine by DNA Polymerases

spinqualitylabelsall models 3v6k, resolution 3.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3v6k is a 6 chain structure with sequence from Sacs2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CA, MG, NA NonStd Res: 2DT, EFG Related: 3v6j, 3v6h, 2c2e, 2br0 Gene: dbh, dpo4, SSO2448 (SACS2) Activity: DNA-directed DNA polymerase, with EC number 2.7.7.7 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [DPO4_SULSO] Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. It is involved in translesional synthesis.[HAMAP-Rule:MF_01113] Publication Abstract from PubMed Damaged goods: The unstable DNA adduct N(2) ,3-ethenoguanine, a product of both exposure to the carcinogen vinyl chloride and of oxidative stress, was built into an oligonucleotide, using an isostere strategy to stabilize the glycosidic bond. This modification was then used to examine the cause of mutations by DNA polymerases, in terms of both the biochemistry of the lesion and a structure of the lesion within a polymerase. Replication of N(2) ,3-Ethenoguanine by DNA Polymerases.,Zhao L, Christov PP, Kozekov ID, Pence MG, Pallan PS, Rizzo CJ, Egli M, Guengerich FP Angew Chem Int Ed Engl. 2012 Apr 4. doi: 10.1002/anie.201109004. PMID:22488769 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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