3wd6 | pdb_00003wd6

Crystal structure of Bombyx mori omega-class glutathione transferase in complex with GSH

spinqualitylabelsall models 3wd6, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3wd6 is a 4 chain structure with sequence from Bommo. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: EDO, GSH, IOD, K, PEG Gene: gsto, GSTo2 (BOMMO) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Glutathione transferases (GSTs) are major phase II detoxification enzymes that play central roles in the defense against various environmental toxicants as well as oxidative stress. Here we report the crystal structure of an Omega-class glutathione transferase of Bombyx mori, bmGSTO, to gain insight into its catalytic mechanism. The structure of bmGSTO complexed with glutathione determined at a resolution of 2.5A reveals that it exists as a dimer and is structurally similar to Omega-class GSTs with respect to its secondary and tertiary structures. Analysis of a complex between bmGSTO and glutathione showed that bound glutathione was localized to the glutathione-binding site (G-site). Site-directed mutagenesis of bmGSTO mutants indicated that amino acid residues Leu62, Lys65, Lys77, Val78, Glu91 and Ser92 in the G-site contribute to catalytic activity. Three-dimensional structure of a Bombyx mori Omega-class glutathione transferase.,Yamamoto K, Suzuki M, Higashiura A, Nakagawa A Biochem Biophys Res Commun. 2013 Sep 6;438(4):588-93. doi:, 10.1016/j.bbrc.2013.08.011. Epub 2013 Aug 11. PMID:23939046 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Glutathione S-transferase 3D structures

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