7uvt | pdb_00007uvt

Kinetically trapped misfolded state of the Tetrahymena ribozyme

spinqualitylabelsall models 7uvt, resolution 3.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7uvt is a 1 chain structure with sequence from Tetrahymena thermophila. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Functional RNAs fold through complex pathways that can contain misfolded "kinetic traps." A complete model of RNA folding requires understanding the formation of these misfolded states, but they are difficult to characterize because of their transient and potentially conformationally dynamic nature. We used cryo-electron microscopy (cryo-EM) to visualize a long-lived misfolded state in the folding pathway of the Tetrahymena thermophila group I intron, a paradigmatic RNA structure-function model system. The structure revealed how this state forms native-like secondary structure and tertiary contacts but contains two incorrectly crossed strands, consistent with a previous model. This incorrect topology mispositions a critical catalytic domain and cannot be resolved locally as extensive refolding is required. This work provides a structural framework for interpreting decades of biochemical and functional studies and demonstrates the power of cryo-EM for the exploration of RNA folding pathways. Cryo-EM reveals an entangled kinetic trap in the folding of a catalytic RNA.,Bonilla SL, Vicens Q, Kieft JS Sci Adv. 2022 Aug 26;8(34):eabq4144. doi: 10.1126/sciadv.abq4144. Epub 2022 Aug, 26. PMID:36026457 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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