7e1w | pdb_00007e1w

Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203

spinqualitylabelsall models 7e1w, resolution 2.67Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7e1w is a 20 chain structure with sequence from Mycobacterium tuberculosis H37Rv and Mycolicibacterium smegmatis MC2 51. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 9Y0, 9YF, CDL, CU, FES, HEA, HEC, HEM, HUU, MQ9, PLM Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [A0R057_MYCS2] Publication Abstract from PubMed Pathogenic mycobacteria pose a sustained threat to global human health. Recently, cytochrome bcc complexes have gained interest as targets for antibiotic drug development. However, there is currently no structural information for the cytochrome bcc complex from these pathogenic mycobacteria. Here, we report the structures of Mycobacterium tuberculosis cytochrome bcc alone (2.68 A resolution) and in complex with clinical drug candidates Q203 (2.67 A resolution) and TB47 (2.93 A resolution) determined by single-particle cryo-electron microscopy. M. tuberculosis cytochrome bcc forms a dimeric assembly with endogenous menaquinone/menaquinol bound at the quinone/quinol-binding pockets. We observe Q203 and TB47 bound at the quinol-binding site and stabilized by hydrogen bonds with the side chains of QcrBThr(313) and QcrBGlu(314), residues that are conserved across pathogenic mycobacteria. These high-resolution images provide a basis for the design of new mycobacterial cytochrome bcc inhibitors that could be developed into broad-spectrum drugs to treat mycobacterial infections. Structure of Mycobacterium tuberculosis cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates.,Zhou S, Wang W, Zhou X, Zhang Y, Lai Y, Tang Y, Xu J, Li D, Lin J, Yang X, Ran T, Chen H, Guddat LW, Wang Q, Gao Y, Rao Z, Gong H Elife. 2021 Nov 25;10. pii: 69418. doi: 10.7554/eLife.69418. PMID:34819223 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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