7xsd | pdb_00007xsd

Cryo-EM structure of RuBisCO assembly intermediate RbcL8Raf18RbcX16

spinqualitylabelsall models 7xsd, resolution 3.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7xsd is a 32 chain structure with sequence from Nostoc sp. PCC 7120 = FACHB-418. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [RBCX_NOSS1] An RbcL-specific chaperone. The central cleft of the RbcX homodimer (RbcX2) binds the C-terminus of an RbcL monomer, stabilizing the C-terminus and probably preventing its reassociation with chaperonin GroEL-ES. At the same time the peripheral region of RbcX2 binds a second RbcL monomer, bridging the RbcL homodimers in the correct orientation. The RbcX2(2)-bound RbcL dimers then assemble into the RbcL8 core (RbcL8-(RbcX2)8). RbcS binding triggers the release of RbcX2.[HAMAP-Rule:MF_00855] When rbcL-rbcX-rbcS or rbcL-rbcS were overexpressed in E.coli no change in reconstituted RuBisCO activity was observed, which suggests RbcX plays no role in RuBisCO assembly in this system (PubMed:8472962). However in PubMed:8472962 E.coli chaperones groL and groS were also overexpressed, which may compensate for lack of rbcX (Probable).

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