8agk | pdb_00008agk

Botulinum neurotoxin subtype A6 cell binding domain in complex with GD1a ganglioside

spinqualitylabelsall models 8agk, resolution 1.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8agk is a 1 chain structure with sequence from Clostridium botulinum str. Iwanei E. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: EDO, GAL, GLA, GLC, NGA, PEG, SIA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [C9WWY7_CLOBO] Publication Abstract from PubMed Clostridium botulinum neurotoxin A (BoNT/A) targets the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex, by cleaving synaptosomal-associated protein of 25 kDa size (SNAP-25). Cleavage of SNAP-25 results in flaccid paralysis due to repression of synaptic transmission at the neuromuscular junction. This activity has been exploited to treat a range of diseases associated with hypersecretion of neurotransmitters, with formulations of BoNT/A commercially available as therapeutics. Generally, BoNT activity is facilitated by three essential domains within the molecule, the cell binding domain (HC), the translocation domain (HN), and the catalytic domain (LC). The HC, which consists of an N-terminal (HCN) and a C-terminal (HCC) subdomain, is responsible for BoNT's high target specificity where it forms a dual-receptor complex with synaptic vesicle protein 2 (SV2) and a ganglioside receptor on the surface of motor neurons. In this study, we have determined the crystal structure of botulinum neurotoxin A6 cell binding domain (HC/A6) in complex with GD1a and describe the interactions involved in ganglioside binding. We also present a new crystal form of wild type HC/A6 (crystal form II) where a large 'hinge motion' between the HCN and HCC subdomains is observed. These structures, along with a comparison to the previously determined wild type crystal structure of HC/A6 (crystal form I), reveals the degree of conformational flexibility exhibited by HC/A6. Crystal Structures of the Clostridium botulinum Neurotoxin A6 Cell Binding Domain Alone and in Complex with GD1a Reveal Significant Conformational Flexibility.,Gregory KS, Newell AR, Mojanaga OO, Liu SM, Acharya KR Int J Mol Sci. 2022 Aug 25;23(17). pii: ijms23179620. doi: 10.3390/ijms23179620. PMID:36077016 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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