4ep7 | pdb_00004ep7

Functional implications from the Cid1 poly(U) polymerase crystal structure

spinqualitylabelsall models 4ep7, resolution 2.28Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4ep7 is a 2 chain structure with sequence from Schizosaccharomyces pombe 972h-. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MG, UTP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [CID1_SCHPO] Involved in cell cycle arrest where in association with crb2/rhp9 and chk1 it inhibits unscheduled mitosis. Publication Abstract from PubMed In eukaryotes, mRNA degradation begins with poly(A) tail removal, followed by decapping, and the mRNA body is degraded by exonucleases. In recent years, the major influence of 3'-end uridylation as a regulatory step within several RNA degradation pathways has generated significant attention toward the responsible enzymes, which are called poly(U) polymerases (PUPs). We determined the atomic structure of the Cid1 protein, the founding member of the PUP family, in its UTP-bound form, allowing unambiguous positioning of the UTP molecule. Our data also suggest that the RNA substrate accommodation and product translocation by the Cid1 protein rely on local and global movements of the enzyme. Supplemented by point mutations, the atomic model is used to propose a catalytic cycle. Our study underlines the Cid1 RNA binding properties, a feature with critical implications for miRNAs, histone mRNAs, and, more generally, cellular RNA degradation. Functional Implications from the Cid1 Poly(U) Polymerase Crystal Structure.,Munoz-Tello P, Gabus C, Thore S Structure. 2012 May 16. PMID:22608966 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Poly(A) RNA polymerase

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