7ybd | pdb_00007ybd
Crystal structure of sliding DNA clamp of Clostridioides difficile
Structural highlights
FunctionQ18C88_CLOD6 Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.[ARBA:ARBA00002266][PIRNR:PIRNR000804] Publication Abstract from PubMedThe sliding DNA clamp is a ring-shaped protein that encircles DNA within its central channel. It binds to multiple proteins, such as DNA polymerases and DNA repair enzymes, and stimulates their enzymatic activities, thereby playing a crucial role in cell survival and proliferation. Accordingly, the bacterial clamp DnaN is considered to be a promising target for bacterial infection therapy. In this regard, three-dimensional structures of DnaN from pathogenic bacteria are essential for the development of chemical compounds with antimicrobial activity. Here, the crystal structure of DnaN from a Gram-positive bacterium Clostridioides difficile, a human pathogen causing infectious diarrhea, has been determined at 2.13 A resolution. Comparison of the structures of DnaN from other bacteria indicates that the structural features of DnaN in terms of overall organization are essentially conserved within Gram-positive and Gram-negative bacteria. However, DnaN from C. difficile has structural differences in the potential binding pocket for partner proteins, implying a non-conventional interaction with its binding partners. Our findings will provide insight into the development of new therapies for C. difficile infection. Crystal structure of the sliding DNA clamp from the Gram-positive anaerobic bacterium Clostridioides difficile.,Hishiki A, Okazaki S, Hara K, Hashimoto H J Biochem. 2022 Sep 27. pii: 6724376. doi: 10.1093/jb/mvac079. PMID:36166824[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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