8dx4 | pdb_00008dx4

Clostridioides difficile R20291 minor pilin - PilW fused with Maltose Binding Protein

spinqualitylabelsall models 8dx4, resolution 2.49Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8dx4 is a 4 chain structure with sequence from Clostridioides difficile R20291 and Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: EDO, EPE, PEG, PGE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A0H3N4W9_CLODC A0A4P1LXE0_SERSF Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Binds maltose and higher maltodextrins.[RuleBase:RU365005] Publication Abstract from PubMed Clostridioides difficile is a Gram-positive bacillus, which is a frequent cause of gastrointestinal infections triggered by the depletion of the gut microbiome. Because of the frequent recurrence of these infections after antibiotic treatment, mechanisms of C. difficile persistence and recurrence, including biofilm formation, are of increasing interest. Previously, our group and others found that type IV pili, filamentous helical appendages polymerized from protein subunits, promoted microcolony and biofilm formation in C. difficile. In Gram-negative bacteria, the ability of type IV pili to mediate bacterial self-association has been explained through interactions between the pili of adjacent cells, but type IV pili from several Gram-negative species are also required for natural competence through DNA-uptake. Here, we report the ability of two C. difficile pilin subunits, PilJ and PilW, to bind to DNA in vitro, as well as the defects in biofilm formation in the pilJ and pilW gene-interruption mutants. Additionally, we have resolved the X-ray crystal structure of PilW, which we use to model possible structural mechanisms for the formation of C. difficile biofilm through interactions between type IV pili and the DNA of the extracellular matrix. Taken together, our results provide further insight into the relationship between type IV pilus function and biofilm formation in C. difficile and, more broadly, suggest that DNA-recognition by type IV pili and related structures may have functional importance beyond DNA-uptake for natural competence. Recognition of extracellular DNA by type IV pili promotes biofilm formation by Clostridioides difficile.,Ronish LA, Sidner B, Yu Y, Piepenbrink KH J Biol Chem. 2022 Sep 2:102449. doi: 10.1016/j.jbc.2022.102449. PMID:36064001 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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