4gex | pdb_00004gex
Structure of a stabilised ceSAS-6 dimer, second crystal form
Structural highlights
FunctionSAS6_CAEEL Required for centrosome duplication. Participates in centriole assembly.[1] [2] Publication Abstract from PubMedCentrioles are evolutionary conserved organelles that give rise to cilia and flagella as well as centrosomes. Centrioles display a characteristic ninefold symmetry imposed by the spindle assembly abnormal protein 6 (SAS-6) family. SAS-6 from Chlamydomonas reinhardtii and Danio rerio was shown to form ninefold symmetric, ring-shaped oligomers in vitro that were similar to the cartwheels observed in vivo during early steps of centriole assembly in most species. Here, we report crystallographic and EM analyses showing that, instead, Caenorhabotis elegans SAS-6 self-assembles into a spiral arrangement. Remarkably, we find that this spiral arrangement is also consistent with ninefold symmetry, suggesting that two distinct SAS-6 oligomerization architectures can direct the same output symmetry. Sequence analysis suggests that SAS-6 spirals are restricted to specific nematodes. This oligomeric arrangement may provide a structural basis for the presence of a central tube instead of a cartwheel during centriole assembly in these species. Caenorhabditis elegans centriolar protein SAS-6 forms a spiral that is consistent with imparting a ninefold symmetry.,Hilbert M, Erat MC, Hachet V, Guichard P, Blank ID, Fluckiger I, Slater L, Lowe ED, Hatzopoulos GN, Steinmetz MO, Gonczy P, Vakonakis I Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11373-8. doi:, 10.1073/pnas.1302721110. Epub 2013 Jun 24. PMID:23798409[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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