8d3p | pdb_00008d3p

Type I-C Cas4-Cas1-Cas2 complex bound to half-site integration intermediate (HSI)

spinqualitylabelsall models 8d3p, resolution 4.26Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8d3p is a 11 chain structure with sequence from Alkalihalobacillus halodurans C-125 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MN, SF4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q9KFX9_HALH5 CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.[HAMAP-Rule:MF_01470] Publication Abstract from PubMed Adaptation in CRISPR-Cas systems immunizes bacteria and archaea against mobile genetic elements. In many DNA-targeting systems, the Cas4-Cas1-Cas2 complex is required for selection and processing of DNA segments containing PAM sequences prior to integration of these "prespacer" substrates as spacers in the CRISPR array. We determined cryo-EM structures of the Cas4-Cas1-Cas2 adaptation complex from the type I-C system that encodes standalone Cas1 and Cas4 proteins. The structures reveal how Cas4 specifically reads out bases within the PAM sequence and how interactions with both Cas1 and Cas2 activate Cas4 endonuclease activity. The Cas4-PAM interaction ensures tight binding between the adaptation complex and the prespacer, significantly enhancing integration of the non-PAM end into the CRISPR array and ensuring correct spacer orientation. Corroborated with our biochemical results, Cas4-Cas1-Cas2 structures with substrates representing various stages of CRISPR adaptation reveal a temporally resolved mechanism for maturation and integration of functional spacers into the CRISPR array. PAM binding ensures orientational integration during Cas4-Cas1-Cas2-mediated CRISPR adaptation.,Dhingra Y, Suresh SK, Juneja P, Sashital DG Mol Cell. 2022 Oct 14. pii: S1097-2765(22)00951-0. doi:, 10.1016/j.molcel.2022.09.030. PMID:36272411 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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