4ija | pdb_00004ija

Structure of S. aureus methicillin resistance factor MecR2

spinqualitylabelsall models 4ija, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4ija is a 2 chain structure with sequence from Staphylococcus aureus subsp. aureus N315. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GOL, K, PO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A0H3JS71_STAAN Transcriptional repressor of xylose-utilizing enzymes.[ARBA:ARBA00002486] Publication Abstract from PubMed Methicillin resistance in Staphylococcus aureus is elicited by the MecI-MecR1-MecA axis encoded by the mec locus. Recently, MecR2 was also identified as the third regulator of mec through binding of the methicillin repressor, MecI. Here we show that plasmid-encoded full-length MecR2 restores resistance in a sensitive S. aureus mecR2 deletion mutant of the resistant strain N315. The crystal structure of MecR2 reveals an N-terminal DNA-binding domain (NDD), an intermediate scaffold domain (ISD), and a C-terminal dimerization domain (CDD) that contributes to oligomerization. The protein shows structural similarity to ROK (from repressors, open-reading frames and kinases) family proteins, which bind DNA and/or sugar molecules. We found that functional cell-based assays of three point mutants affecting residues participating in sugar binding in ROK proteins had no effect on the resistance phenotype. By contrast, MecR2 bound short double-stranded DNA oligonucleotides non-specifically and a deletion mutant affecting the NDD showed a certain effect on activity, thus contributing to resistance less than the wild-type protein. Similarly, a deletion mutant, in which a flexible segment of ISD had been replaced by four glycines, significantly reduced MecR2 function, thus indicating that this domain may likewise be required for activity. Taken together, these results provide the structural basis for the activity of methicillin anti-repressor, MecR2, which would sequester MecI away from its cognate promoter region and facilitate its degradation. Structure-function studies of the staphylococcal methicillin resistance anti-repressor, MecR2.,Arede P, Botelho T, Guevara T, Uson I, Oliveira DC, Gomis-Ruth FX J Biol Chem. 2013 Jun 3. PMID:23733184 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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