7uin

CryoEM Structure of an Group II Intron Retroelement

spinqualitylabelsall models 7uin, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7uin is a 3 chain structure with sequence from Eubacterium rectale. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MG, NH4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A173ZME3_9FIRM Publication Abstract from PubMed Group II introns are ribozymes that catalyze their self-excision and function as retroelements that invade DNA. As retrotransposons, group II introns form ribonucleoprotein (RNP) complexes that roam the genome, integrating by reversal of forward splicing. Here we show that retrotransposition is achieved by a tertiary complex between a structurally elaborate ribozyme, its protein mobility factor, and a structured DNA substrate. We solved cryo-electron microscopy structures of an intact group IIC intron-maturase retroelement that was poised for integration into a DNA stem-loop motif. By visualizing the RNP before and after DNA targeting, we show that it is primed for attack and fits perfectly with its DNA target. This study reveals design principles of a prototypical retroelement and reinforces the hypothesis that group II introns are ancient elements of genetic diversification. Structures of a mobile intron retroelement poised to attack its structured DNA target.,Chung K, Xu L, Chai P, Peng J, Devarkar SC, Pyle AM Science. 2022 Nov 11;378(6620):627-634. doi: 10.1126/science.abq2844. Epub 2022 , Nov 10. PMID:36356138 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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