7vru | pdb_00007vru

Crystal structure of PacII_M1M2S-DNA-SAH complex

spinqualitylabelsall models 7vru, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7vru is a 5 chain structure with sequence from Pseudomonas alcaligenes and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MSE, SAH Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A142ISP2_PSEAC Publication Abstract from PubMed Type I restriction-modification systems help establish the prokaryotic DNA methylation landscape and provide protection against invasive DNA. In addition to classical m6A modifications, non-canonical type I enzymes catalyze both m6A and m4C using alternative DNA-modification subunits M1 and M2. Here, we report the crystal structures of the non-canonical PacII_M1M2S methyltransferase bound to target DNA and reaction product S-adenosylhomocysteine in a closed clamp-like conformation. Target DNA binds tightly within the central tunnel of the M1M2S complex and forms extensive contacts with all three protein subunits. Unexpectedly, while the target cytosine properly inserts into M2's pocket, the target adenine (either unmethylated or methylated) is anchored outside M1's pocket. A unique asymmetric catalysis is established where PacII_M1M2S has precisely coordinated the relative conformations of different subunits and evolved specific amino acids within M2/M1. This work provides insights into mechanisms of m6A/m4C catalysis and guidance for designing tools based on type I restriction-modification enzymes. Molecular insights into DNA recognition and methylation by non-canonical type I restriction-modification systems.,Zhu J, Gao Y, Wang Y, Zhan Q, Feng H, Luo X, Li P, Liu S, Hou H, Gao P Nat Commun. 2022 Oct 27;13(1):6391. doi: 10.1038/s41467-022-34085-z. PMID:36302770 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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