8age | pdb_00008age

Structure of yeast oligosaccharylransferase complex with acceptor peptide bound

spinqualitylabelsall models 8age, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8age is a 9 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 323, BMA, CPL, KZB, MAN, MG, NAG, PTY Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A6A5Q0M3_YEASX Publication Abstract from PubMed Oligosaccharyltransferase (OST) is the central enzyme of N-linked protein glycosylation. It catalyzes the transfer of a pre-assembled glycan, GlcNAc(2)Man(9)Glc(3), from a dolichyl-pyrophosphate donor to acceptor sites in secretory proteins in the lumen of the endoplasmic reticulum. Precise recognition of the fully assembled glycan by OST is essential for the subsequent quality control steps of glycoprotein biosynthesis. However, the molecular basis of the OST-donor glycan interaction is unknown. Here we present cryo-EM structures of S. cerevisiae OST in distinct functional states. Our findings reveal that the terminal glucoses (Glc(3)) of a chemo-enzymatically generated donor glycan analog bind to a pocket formed by the non-catalytic subunits WBP1 and OST2. We further find that binding either donor or acceptor substrate leads to distinct primed states of OST, where subsequent binding of the other substrate triggers conformational changes required for catalysis. This alternate priming allows OST to efficiently process closely spaced N-glycosylation sites. Molecular basis for glycan recognition and reaction priming of eukaryotic oligosaccharyltransferase.,Ramirez AS, de Capitani M, Pesciullesi G, Kowal J, Bloch JS, Irobalieva RN, Reymond JL, Aebi M, Locher KP Nat Commun. 2022 Nov 26;13(1):7296. doi: 10.1038/s41467-022-35067-x. PMID:36435935 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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