4n5x | pdb_00004n5x

Crystal structure of N-terminal calmodulin-like Calcium sensor of human mitochondrial ATP-Mg/Pi carrier SCaMC1

spinqualitylabelsall models 4n5x, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4n5x is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CA, PEG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function SCMC1_HUMAN Calcium-dependent mitochondrial solute carrier. Mitochondrial solute carriers shuttle metabolites, nucleotides, and cofactors through the mitochondrial inner membrane. May act as a ATP-Mg/Pi exchanger that mediates the transport of Mg-ATP in exchange for phosphate, catalyzing the net uptake or efflux of adenine nucleotides into or from the mitochondria. Publication Abstract from PubMed The mitochondrial carriers play essential roles in energy metabolism. The short Ca(2+)-binding mitochondrial carrier (SCaMC) transports ATP-Mg in exchange for Pi and is important for activities that depend on adenine nucleotides. SCaMC adopts, in addition to the transmembrane domain (TMD) that transports solutes, an extramembrane N-terminal domain (NTD) that regulates solute transport in a Ca(2+)-dependent manner. Crystal structure of the Ca(2+)-bound NTD reveals a compact architecture in which the functional EF hands are sequestered by an endogenous helical segment. Nuclear magnetic resonance (NMR) relaxation rates indicated that removal of Ca(2+) from NTD results in a major conformational switch from the rigid and compact Ca(2+)-bound state to the dynamic and loose apo state. Finally, we showed using surface plasmon resonance and NMR titration experiments that free apo NTDs could specifically interact with liposome-incorporated TMD, but that Ca(2+) binding drastically weakened the interaction. Our results together provide a molecular explanation for Ca(2+)-dependent ATP-Mg flux in mitochondria. A Self-Sequestered Calmodulin-like Ca(2+) Sensor of Mitochondrial SCaMC Carrier and Its Implication to Ca(2+)-Dependent ATP-Mg/Pi Transport.,Yang Q, Bruschweiler S, Chou JJ Structure. 2014 Feb 4;22(2):209-17. doi: 10.1016/j.str.2013.10.018. Epub 2013 Dec, 12. PMID:24332718 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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