7drs | pdb_00007drs

Structure of SspE_40224

spinqualitylabelsall models 7drs, resolution 3.42Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7drs is a 4 chain structure with sequence from Streptomyces yokosukanensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A6I8WFL9_9ACTN Publication Abstract from PubMed DNA phosphorothioate (PT) modification, with a nonbridging phosphate oxygen substituted by sulfur, represents a widespread epigenetic marker in prokaryotes and provides protection against genetic parasites. In the PT-based defense system Ssp, SspABCD confers a single-stranded PT modification of host DNA in the 5'-C(PS)CA-3' motif and SspE impedes phage propagation. SspE relies on PT modification in host DNA to exert antiphage activity. Here, structural and biochemical analyses reveal that SspE is preferentially recruited to PT sites mediated by the joint action of its N-terminal domain (NTD) hydrophobic cavity and C-terminal domain (CTD) DNA binding region. PT recognition enlarges the GTP-binding pocket, thereby increasing GTP hydrolysis activity, which subsequently triggers a conformational switch of SspE from a closed to an open state. The closed-to-open transition promotes the dissociation of SspE from self PT-DNA and turns on the DNA nicking nuclease activity of CTD, enabling SspE to accomplish self-nonself discrimination and limit phage predation, even when only a small fraction of modifiable consensus sequences is PT-protected in a bacterial genome. Nicking mechanism underlying the DNA phosphorothioate-sensing antiphage defense by SspE.,Gao H, Gong X, Zhou J, Zhang Y, Duan J, Wei Y, Chen L, Deng Z, Wang J, Chen S, Wu G, Wang L Nat Commun. 2022 Nov 9;13(1):6773. doi: 10.1038/s41467-022-34505-0. PMID:36351933 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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