7y8u | pdb_00007y8u

Crystal structure of AlbEF homolog from Quasibacillus thermotolerans

spinqualitylabelsall models 7y8u, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7y8u is a 2 chain structure with sequence from Quasibacillus thermotolerans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: EDO, PO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Subtilosin A is a sactipeptide bacteriocin produced by Bacillus subtilis strain 168, containing intramolecular thioether bonds and a head-to-tail macrocyclic peptide bond. Macrocyclization is presumably catalyzed by AlbE and AlbF proteins encoded by the subtilosin A biosynthesis gene cluster. However, the underlying mechanism of macrocyclization remains uncertain as the tertiary structures of the proteins are undetermined. Here, we report the crystal structures of AlbE and AlbF homologs in Quasibacillus thermotolerans, wherein the subtilosin biosynthesis gene cluster is highly conserved. Structural analysis and pull-down assays revealed that AlbE and AlbF form heterodimeric complexes. Although the AlbEF complex shows structural similarity to M16B family metalloproteases, the substrate-binding chamber is shallower and more open than the other M16B family proteins. The chamber surface showed electrostatic complementarity to the precursor of subtilosin. Our findings provide insights into the role of AlbEF in metalloprotease catalysis and macrocyclic peptide bond formation. Crystal structure of the AlbEF complex involved in subtilosin A biosynthesis.,Ishida K, Nakamura A, Kojima S Structure. 2022 Dec 1;30(12):1637-1646.e3. doi: 10.1016/j.str.2022.10.002. Epub , 2022 Oct 26. PMID:36302388 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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