4o88 | pdb_00004o88

Crystal structure of a C-tagged Nuclease

spinqualitylabelsall models 4o88, resolution 2.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4o88 is a 2 chain structure with sequence from Millerozyma acaciae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CL, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q707V3_9ASCO Publication Abstract from PubMed tRNA restriction by anticodon nucleases underlies cellular stress responses and self-nonself discrimination in a wide range of taxa. Anticodon breakage inhibits protein synthesis, which, in turn, results in growth arrest or cell death. The eukaryal ribotoxin PaT secreted by Pichia acaciae inhibits growth of Saccharomyces cerevisiae via cleavage of tRNA(Gln(UUG)). We find that recombinant PaT incises a synthetic tRNA(Gln(UUG)) stem-loop RNA by transesterification at a single site 3' of the wobble uridine, yielding 2',3'-cyclic phosphate and 5'-OH ends. Incision is suppressed by replacement of the wobble nucleobase with adenine or guanine. The crystal structure of PaT reveals a distinctive fold and active site, essential components of which are demonstrated by mutagenesis. Pichia acaciae evades self-toxicity via a distinctive intracellular immunity protein, ImmPaT, which binds PaT and blocks nuclease activity. Our results highlight the evolutionary diversity of tRNA restriction and immunity systems. Structure, mechanism, and specificity of a eukaryal tRNA restriction enzyme involved in self-nonself discrimination.,Chakravarty AK, Smith P, Jalan R, Shuman S Cell Rep. 2014 Apr 24;7(2):339-47. doi: 10.1016/j.celrep.2014.03.034. Epub 2014, Apr 13. PMID:24726365 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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