7z8a | pdb_00007z8a
Sam68
Structural highlights
FunctionPublication Abstract from PubMedSpinal muscular atrophy (SMA) is a neurodegenerative disease caused by the absence of a functional copy of the Survival of Motor Neuron 1 gene (SMN1). The nearly identical paralog, SMN2, cannot compensate for the loss of SMN1 because exon 7 is aberrantly skipped from most SMN2 transcripts, a process mediated by synergistic activities of Sam68/KHDRBS1 and hnRNP A1. This results in the production of a truncated, non-functional protein that is rapidly degraded. Here we present several crystal structures of Sam68 RNA-binding domain (RBD). Sam68-RBD forms stable symmetric homodimers by antiparallel association of helices alpha3 from two monomers. However, the details of domain organization and the dimerization interface differ significantly from previously characterized homologs. We demonstrate that Sam68 and hnRNP A1 can simultaneously bind proximal motifs within the central region of SMN2(ex7). Further, we show that the RNA-binding pockets of the two proteins are close to each other in their heterodimeric complex and identify contact residues using crosslinking-mass spectrometry. We present a model of the ternary Sam68.SMN2(ex7).hnRNP A1 complex that reconciles all available information on SMN1/2 splicing. Our findings have important implications for the etiology of SMA and open new avenues for the design of novel therapeutics to treat splicing diseases. This article is protected by copyright. All rights reserved. Structure and function analysis of Sam68 and hnRNP A1 synergy in the exclusion of exon 7 from SMN2 transcripts.,Nadal M, Anton R, Dorca-Arevalo J, Estebanez-Perpina E, Tizzano EF, Fuentes-Prior P Protein Sci. 2022 Dec 22:e4553. doi: 10.1002/pro.4553. PMID:36560896[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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