7cmj | pdb_00007cmj

Crystal structure of L.donovani Hypoxanthine-guanine phosphoribosyl transferase (HGPRT)

spinqualitylabelsall models 7cmj, resolution 2.76Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7cmj is a 2 chain structure with sequence from Leishmania donovani BPK282A1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: BA, CL, MG, PEG, PG4, PO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function E9BF84_LEIDB Publication Abstract from PubMed Purine bases, synthesized de novo or recycled through the salvage pathway, are precursors of nucleotide synthesis and are essential in a variety of physiological processes including cell division, growth, signaling, energy metabolism and synthesis of vitamins/co-factor. The protozoan kinetoplastid parasites including Leishmania cannot synthesize de novo and rely solely on the purine salvage pathway, recycling the degraded products of nucleic acid metabolism. Enzymes of this pathway are thus of therapeutic importance. The enzyme Hypoxanthine-guanine phosphoribosyl transferase (HGPRT) (EC 2.4.2.8) plays a central role in this pathway, converting the purine base to its monophosphate product. Towards the elucidation of its role, we have cloned, expressed, purified and determined the crystal structure of L. donovani HGPRT at 2.76 A. Comparative structural analysis with the human homolog indicates differences in oligomer association. Comparative analyses identify insertions in the human homolog sequence in the tetramer interface. The results suggest that this difference can be exploited for therapeutic approaches. The L.donovani Hypoxanthine-guanine phosphoribosyl transferase (HGPRT) oligomer is distinct from the human homolog.,Parihar PS, Pratap JV Biochem Biophys Res Commun. 2020 Nov 19;532(4):499-504. doi: , 10.1016/j.bbrc.2020.08.052. Epub 2020 Aug 30. PMID:32873391 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Phosphoribosyltransferase 3D structures

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