2ls6 | pdb_00002ls6

Solution NMR Structure of a Non-canonical galactose-binding CBM32 from Clostridium perfringens

spinqualitylabelsall models 2ls6 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2ls6 is a 1 chain structure with sequence from Clostridium perfringens ATCC 13124. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A0H2YRL1_CLOP1 Publication Abstract from PubMed Carbohydrate-binding modules (CBMs) are ancillary modules commonly associated with carbohydrate-active enzymes (CAZymes) that function to mediate the adherence of the parent enzyme to its carbohydrate substrates. CBM family 32 (CBM32) is one of the most diverse CBM families, whose members are commonly found in bacterial CAZymes that modify eukaryotic glycans. One such example is the putative mu-toxin, CpGH84A, of the family 84 glycoside hydrolases, which comprises an N-terminal putative beta-N-acetylglucosaminidase catalytic module and four tandem CBM32s. Here, we report a unique mode of galactose recognition by the first CBM32, CBM32-1 from CpGH84A. Solution NMR-based analyses of CpGH84A CBM32-1 indicate a divergent subset of residues, located in ordered loops at the apex of the CBM, conferring specificity for the galacto-configured sugars galactose, GalNAc, and LacNAc that differs from those of the canonical galactose-binding CBM32s. This study showcases the impressive variability in ligand binding by this CBM family and offers insight into the growing role of these modules in the interaction of CAZymes with eukaryotic glycans. An Unusual Mode of Galactose Recognition by a Family 32 Carbohydrate-Binding Module.,Grondin JM, Chitayat S, Ficko-Blean E, Houliston S, Arrowsmith CH, Boraston AB, Smith SP J Mol Biol. 2013 Dec 8. pii: S0022-2836(13)00744-4. doi:, 10.1016/j.jmb.2013.11.029. PMID:24326248 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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