8b97 | pdb_00008b97

N-terminal beta-trefoil lectin domain of the Laccaria bicolor lectin in complex with N-acetyl-lactosamine

spinqualitylabelsall models 8b97, resolution 0.97Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8b97 is a 1 chain structure with sequence from Laccaria bicolor S238N-H53. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GAL, NAG, PRD_900019 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B0D650_LACBS Publication Abstract from PubMed Lectins from fruiting bodies are a diverse group of sugar-binding proteins from mushrooms that face the biologically relevant challenge of discriminating self- from non-self carbohydrate structures, therefore providing a basis for an innate defence system. Such a system entails both detection and destruction of invaders and/or feeders, and in contrast to more complex organisms with immense immune systems, these two functions normally rely on multitasking lectins, namely, lectins with different functional modules. Here, we present a novel fungal lectin, LBL, from the basidiomycete Laccaria bicolor. Using a diverse set of biophysical techniques, we unveil the fine details of the sugar-binding specificity of the N-terminal beta-trefoil of LBL (LBL(152)), whose structure has been determined at the highest resolution so far reported for such a fold. LBL(152) binds complex poly-N-Acetyllactosamine polysaccharides and also robust LBL(152) binding to Caenorhabditis elegans and Drosophila melanogaster cellular extracts was detected in microarray assays, with a seeming preference for the fruit fly adult and pupa stages over the larva stage. Prediction of the structure of the C-terminal part of LBL with AlphaFold reveals a tandem repeat of two structurally almost identical domains of around 110 amino acids each, despite sharing low sequence conservation. Atomic crystal structure and sugar specificity of a beta-trefoil lectin domain from the ectomycorrhizal basidiomycete Laccaria bicolor.,Acebron I, Campanero-Rhodes MA, Solis D, Menendez M, Garcia C, Lillo MP, Mancheno JM Int J Biol Macromol. 2023 Feb 7;233:123507. doi: 10.1016/j.ijbiomac.2023.123507. PMID:36754262 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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