7zhc | pdb_00007zhc

Moss spermine/spermidine acetyl transferase (PpSSAT) in complex with AcetylCoA and polyethylen glycol

spinqualitylabelsall models 7zhc, resolution 1.82Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7zhc is a 2 chain structure with sequence from Physcomitrium patens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 1PE, ACO, GOL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A2K1KKM6_PHYPA Publication Abstract from PubMed Polyamines such as spermidine and spermine (Spm) are essential regulators of cell growth, differentiation, maintenance of ion balance and abiotic stress tolerance. Their levels are controlled by the spermidine/spermine N(1) -acetyltransferase (SSAT) via acetylation to promote either their degradation or export outside the cell as shown in mammals. Plant genomes contain at least one gene coding for SSAT, also named NATA for N-AcetylTransferase Activity. Combining kinetics, HPLC-MS and crystallography, here we show that three plant SSATs, one from the lower plant moss Physcomitrium patens and two from the higher plant Zea mays, acetylate various aliphatic polyamines and two amino acids lysine (Lys) and ornithine (Orn). Thus, plant SSATs exhibit a broad substrate specificity, unlike more specific human SSATs (hSSATs) as hSSAT1 targets polyamines whereas hSSAT2 acetylates Lys and thiaLys. The crystal structures of two PpSSAT ternary complexes, one with Lys and coenzyme A (CoA), the other with acetyl-CoA and polyethylene glycol (mimicking Spm) reveal a different binding mode for polyamine versus amino acid substrates accompanied by structural rearrangements of both the coenzyme and the enzyme. Two arginine residues, unique among plant SSATs, hold the carboxyl group of amino acid substrates. The most abundant acetylated compound accumulated in moss was N(6) -acetyl-Lys whereas N(5) -acetyl-Orn, known as toxic for aphids, was found in maize. Both plant species contain very low levels of acetylated polyamines. The study provides a detailed biochemical and structural basis of plant SSAT enzymes that can acetylate a wide range of substrates and likely play various roles in planta. Biochemical and structural basis of polyamine, lysine and ornithine acetylation catalyzed by spermine/spermidine N-acetyl transferase in moss and maize.,Belicek J, Luptakova E, Kopecny D, Frommel J, Vigouroux A, Cavar Zeljkovic S, Jagic F, Briozzo P, Kopecny DJ, Tarkowski P, Nisler J, De Diego N, Morera S, Kopecna M Plant J. 2023 Feb 14. doi: 10.1111/tpj.16148. PMID:36786691 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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