2n8x | pdb_00002n8x

Solution structure of LptE from Pseudomonas Aerigunosa

spinqualitylabelsall models 2n8x Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2n8x is a 1 chain structure with sequence from Pseudomonas aeruginosa PAO1. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q9HX32_PSEAE Together with LptD, is involved in the assembly of lipopolysaccharide (LPS) at the surface of the outer membrane. Required for the proper assembly of LptD. Binds LPS and may serve as the LPS recognition site at the outer membrane.[HAMAP-Rule:MF_01186][SAAS:SAAS00074338] Publication Abstract from PubMed LptE is an outer membrane (OM) lipoprotein found in Gram-negative bacteria, where it forms a complex with the beta-barrel lipopolysaccharide (LPS) transporter LptD. The LptD/E complex plays a key role in OM biogenesis, by translocating newly synthesized LPS molecules from the periplasm into the external leaflet of the asymmetric OM during cell growth. The LptD/E complex in Pseudomonas aeruginosa (Pa) is a target for macrocyclic beta-hairpin-shaped peptidomimetic antibiotics, which inhibit the transport of LPS to the cell surface. So far, the three-dimensional structure of the Pa LptD/E complex and the mode of interaction with these antibiotics are unknown. Here, we report the solution structure of a Pa LptE derivative lacking the N-terminal lipid membrane anchor, determined by multidimensional solution nuclear magnetic resonance (NMR) spectroscopy. The structure reveals a central five-stranded beta-sheet against which pack a long C-terminal and a short N-terminal alpha-helix, as found in homologues of LptE from other Gram-negative bacteria. One unique feature is an extended C-terminal helix in Pa LptE, which in a model of the Pa LptD/E complex appears to be long enough to contact the periplasmic domain of LptD. Chemical shift mapping experiments suggest only weak interactions occur between LptE and the oligosaccharide chains of LPS. The NMR structure of Pa LptE will be valuable for more detailed structural studies of the LptD/E complex from P. aeruginosa. Solution Structure and Dynamics of LptE from Pseudomonas aeruginosa.,Moehle K, Kocherla H, Bacsa B, Jurt S, Zerbe K, Robinson JA, Zerbe O Biochemistry. 2016 May 31;55(21):2936-43. doi: 10.1021/acs.biochem.6b00313. Epub , 2016 May 19. PMID:27166502 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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