3qob | pdb_00003qob

Mechanical Coupling Controls Cooperative Ligand Binding in a Homodimeric Hemoglobin

spinqualitylabelsall models 3qob, resolution 1.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3qob is a 2 chain structure with sequence from Anadara inaequivalvis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CMO, HEM Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function GLB1_ANAIN Publication Abstract from PubMed Here we present a meta-analysis of a large collection of static structures of a protein in the Protein Data Bank in order to extract the progression of structural events during protein function. We apply this strategy to the homodimeric hemoglobin HbI from Scapharca inaequivalvis. We derive a simple dynamic model describing how binding of the first ligand in one of the two chemically identical subunits facilitates a second binding event in the other partner subunit. The results of our ultrafast time-resolved crystallographic studies support this model. We demonstrate that HbI functions like a homodimeric mechanical device, such as pliers or scissors. Ligand-induced motion originating in one subunit is transmitted to the other via conserved pivot points, where the E and F' helices from two partner subunits are "bolted" together to form a stable dimer interface permitting slight relative rotation but preventing sliding. Cooperative macromolecular device revealed by meta-analysis of static and time-resolved structures.,Ren Z, Srajer V, Knapp JE, Royer WE Jr Proc Natl Acad Sci U S A. 2011 Dec 14. PMID:22171006 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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