4v6v | pdb_00004v6v

Tetracycline resistance protein Tet(O) bound to the ribosome

Loading... Status: waiting for Mol* initialization Structural highlights 4v6v is a 10 chain structure with sequence from Escherichia coli. This structure supersedes the now removed PDB entries 3j36 and 3j37. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 1MG, 2MA, 2MG, 3TD, 4OC, 4SU, 5MC, 5MU, 6MZ, 7MG, H2U, MA6, OMC, OMG, OMU, PSU, UR3 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RL20_ECOLI One of the primary rRNA binding proteins, it binds close to the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly.[HAMAP-Rule:MF_00382] Publication Abstract from PubMed Tetracycline resistance protein Tet(O), which protects the bacterial ribosome from binding the antibiotic tetracycline, is a translational GTPase with significant similarity in both sequence and structure to the elongation factor EF-G. Here, we present an atomic model of the Tet(O)-bound 70S ribosome based on our cryo-electron microscopic reconstruction at 9.6-A resolution. This atomic model allowed us to identify the Tet(O)-ribosome binding sites, which involve three characteristic loops in domain 4 of Tet(O). Replacements of the three amino-acid tips of these loops by a single glycine residue result in loss of Tet(O)-mediated tetracycline resistance. On the basis of these findings, the mechanism of Tet(O)-mediated tetracycline resistance can be explained in molecular detail. Mechanism of tetracycline resistance by ribosomal protection protein Tet(O).,Li W, Atkinson GC, Thakor NS, Allas U, Lu CC, Chan KY, Tenson T, Schulten K, Wilson KS, Hauryliuk V, Frank J Nat Commun. 2013;4:1477. doi: 10.1038/ncomms2470. PMID:23403578 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosome 3D structures Transfer RNA (tRNA)

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