4wwu | pdb_00004wwu
Structure of Mex67:Mtr2
Structural highlights
FunctionMEX67_YEAST Involved in the export of mRNA from the nucleus to the cytoplasm.[1] Publication Abstract from PubMedThe Mex67:Mtr2 complex is the principal yeast nuclear export factor for bulk mRNA and also contributes to ribosomal subunit export. Mex67 is a modular protein constructed from four domains (RRM, LRR, NTF2-like and UBA) that have been thought to be joined by flexible linkers like beads on a string, with the RRM and LRR domains binding RNAs and the NTF2-like and UBA domains binding FG-nucleoporins to facilitate movement through nuclear pores. Here, we show that the NTF2-like domain from Saccharomyces cerevisiae Mex67:Mtr2 also contributes to RNA binding. Moreover, the 3.3 A resolution crystal structure of the Mex67DeltaUBA:Mtr2 complex, supplemented with small angle X-ray scattering data, indicated that the LRR domain has a defined spatial relationship to the Mex67NTF2L:Mtr2 region. Conversely, the RRM domain and especially the UBA domain are more mobile. The conformation assumed by the LRR and NTF2-like domains results in clusters of positively-charged residues on each becoming arranged to form a continuous interface for binding RNA on the opposite side of the complex to the region that interacts with FG-nucleoporins to facilitate passage through nuclear pores. Domain organization within the nuclear export factor Mex67:Mtr2 generates an extended mRNA binding surface.,Aibara S, Valkov E, Lamers M, Stewart M Nucleic Acids Res. 2015 Jan 23. pii: gkv030. PMID:25618852[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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