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Human B-cell Antigen Receptor: IgM BCR
ContentsIntroductionThe adaptive immune response possessed by vertebrate animals owes much of its function to B cells. These specialized immune cells produce antibodies and immunoglobulins (Ig), the membrane bound equivalent to antibodies. B cells can produce a variety of Ig compounds including IgG, IgA, IgE, IgD, and IgM. These antibodies and Ig compounds bind to specific compounds called antigens. When an IgM combines with a B cell receptor (BCR), it can then send a signal in the form of a conformational change through the B cell membrane to stimulate the production of more antibodies that recognize that antigen. [1] The structure of the IgM BCR complex was determined by two research groups using Cryo EM. They also determined the structure of IgG. [2], [3] Structure The IgM BCR consists of six separate chains (Figure 1) that make up three main domains in the molecule. A depiction of the IgM colored by domain shows two heavy and two light chains that together form the Fab region, or variable fragment at the top of the molecule where the antigen binding sites are located. The two heavy chains extend below the Fab region through the Fc region and eventually connect to the Igα/β heterodimer to form the transmembrane region which anchors the overall complex to the B cell. The overall structure, expression, and function of the IgM BCR is strongly influenced by the transmembrane region in which Ig α/β interactions as a heterodimer influence cell surface expression, receptor assembly, and effective signal transduction. [4], [5]. In each domain, interactions between individual chains are important to understand the complex as a whole. All future 3D depictions will be colored by chain as in Figure 1. Transmembrane RegionThe IgM BCR is anchored to B-cell membranes through the transmembrane region which is broken up into both extracellular and integral domains which sit on top of or span through the membrane, respectively (Figure 1). IgM BCR assembly requires dimerization of the Igα and Igβ subunits which embed within the B-cell membrane. [4] The Igα and Igβ heterodimer dimerizes within the extracellular region with a disulfide bridge. Additional dimerization occurs within the integral region via a hydrogen bond; the residues involved have not been confirmed. Although the mechanism of disulfide bridge formation is still unknown, extracellular glycosylation via N-linked asparagine glycosyl groups (NAGs) on various residues in the extracellular region of both the Igα and and Igβ chains is hypothesized to help facilitate this process by recruiting Chaperone proteins to optimize the folding process. [6] Past studies with human and viral proteins have shown that the presence of NAGs not only facilitate the rapid formation of disulfide bridges, but also ensure correct location. [7], [8] The recruited chaperone proteins will typically remain bound to the Igα and Igβ subunits until dimerization occurs. [5] After Igα and Igβ dimerization, the transmembrane helices of the heavy chains can embed within the B-cell membrane and will intertwine with the Igα and Igβ chains. [4] The side chains of this 4-pass integral helix structure are primarily hydrophobic side chains that allow for interactions with the hydrophobic tails in the phospholipid bilayer. The four helices (Figure 2) are primarily held together through hydrophobic interactions; however, a a few polar residues are included on the interior of the helix structure which interact with a few polar residues on the Igα and Igβ chains. [5]  Within the transmembrane region, heavy chain A and heavy chain B associate (Figure 1) asymmetrically to facilitate intracellular signaling cascades. The transmembrane heavy chain interface allows them to pack together via Van der Waals contacts, but there are also prominent hydrogen bonds between each chain. More specifically, the hydroxyl group from Ser584 on heavy chain A donates a hydrogen bond to Ser584 and to Ser588 on heavy chain B. This creates a bifurcated hydrogen bond, essentially forming a “fork” between the two chains to help stabilize them and maintain the transmission of the signal once the cell is activated. Because transmembrane Ig molecules cannot efficiently initiate the signal cascade, they must associate with the Igα and Igβ chains within the BCR. [2] Furthermore, both the Igα and Igβ chains have cytoplasmic tails that extend into the B cell (Figure 1). Each of these tails contain an ITAM region to facilitate signal transduction (Figure 4). [3] The structures of the ITAM regions have not yet been determined. Fc RegionThe constant region of IgM is made up of the two heavy chains. These heavy chains form a bridge connecting the FAB region or variable region to the transmembrane region (Figure 1). They also act as a wire that the variable region can send a signal through to the transmembrane region as a mechanical change. Extracellular transmembrane interactions help hold the heavy chains and Igα/Igβ chains together in the extracellular portion of the transmembrane region. Because a conformational change occurs throughout the entirety of the IgM-BCR complex, the Fc region must be able to tolerate the contortion of the molecule as the antigen binds. In constant region two, which is located at the start of the Fc region, heavy chain A and heavy chain B make a disulfide bridge to stabilize the IgM-BCR and drive downstream signaling. To maximize the Fc region’s signal transduction efficiency and Van der Waals contacts, constant region two of heavy chain A makes an asymmetrical association with constant region three of heavy chain B to create a heavy chain interface. More specifically, Arg243 and Arg251 residues from heavy chain A donate three hydrogen bonds to Leu433, Thr431, and Asp376 residues on heavy chain B. Furthermore, Leu313 of heavy chain A accepts a hydrogen bond from Thr429 on heavy chain B. [3] Fab RegionThe Fab region of the antibody is where antigen recognition occurs upon binding (Figure 1). On each arm is one heavy (A/B) and one light (A/B) chain, both containing domains identical to their respective counterparts. Repeats of β-sandwiches form the constant and variable domains within the Fab region as antigen recognition occurs at the variable domain while the constant domain connects it to the rest of the IgM complex. Because the Fab region of IgM is poorly resolved, a structural analysis of an HIV neutralizing antibody called VCR01 was performed to approximate where an antigen would bind to at the variable region. [9] The IgM-BCR contains areas referred to as complementary-determining regions(CDRs), which are where the antigen makes contact with the antibody on the Fab domain. Figure 2 depicts this as a surface representation given that the specific residues within the antigen-binding motif are unknown. Due to the poor resolution of the Fab region, specific side chain interactions between the heavy (A/B) and light (A/B) chains have not been determined. It is estimated that each β-sandwich contains one disulfide bridge with additional hydrogen bonds. The heavy-light chain interface shows how the four heavy and light chain β-sandwiches fit together. The Fab region heavy chains attach to the Fc region heavy chains, before continuing down into the intracellular domain to interact with the Igα/Igβ subunits. The light chains (A/B) however are only connected to the heavy chains (A/B) within the Fab region, thus have no contact with the Igα/Igβ heterodimer. 
Signal TransductionThe diagram in Figure 4 depicts the initial process of B cell activation by the antigen binding to the antibody at the Fab region. The underlying mechanism for signal transduction is unknown but it is speculated to operate under what is known as the conserved assembly mechanism. [3] This means that upon antigen binding, BCRs on the surface of the cell begin to cluster to cause the phosphorylation of the immunoreceptor tyrosine-based activation motifs located in Igα and Igβ. In its “off” state, the constant region 4 of heavy chain B overlaps the extracellular components of Igα and Igβ. As the antigen binds, it induces a conformational change to release the overlap and allow for clustering about the BCR. Now, in its “on” state the phosphorylation of the ITAM region (observed in figure 4 as conserved phosphorylated tyrosine residues) within the intracellular tails of Igα and Igβ drives downstream kinase activity to continue to process of signal cascading. 
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References
Student Contributors
DeTonyeá Dickson, Allison Goss, Jackson Payton
