8sbb | pdb_00008sbb

Cryo-EM structure of FtAlkB

spinqualitylabelsall models 8sbb, resolution 3.59Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8sbb is a 2 chain structure with sequence from Fontimonas thermophila and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: D12, FE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A1I2KHB9_9GAMM Publication Abstract from PubMed Alkanes are the most energy-rich form of carbon and are widely dispersed in the environment. Their transformation by microbes represents a key step in the global carbon cycle. Alkane monooxygenase (AlkB), a membrane-spanning metalloenzyme, converts straight chain alkanes to alcohols in the first step of the microbially-mediated degradation of alkanes, thereby playing a critical role in the global cycling of carbon and the bioremediation of oil. AlkB biodiversity is attributed to its ability to oxidize alkanes of various chain lengths, while individual AlkBs target a relatively narrow range. Mechanisms of substrate selectivity and catalytic activity remain elusive. Here we report the cryo-EM structure of AlkB, which provides a distinct architecture for membrane enzymes. Our structure and functional studies reveal an unexpected diiron center configuration and identify molecular determinants for substrate selectivity. These findings provide insight into the catalytic mechanism of AlkB and shed light on its function in alkane-degrading microorganisms. Structure and mechanism of the alkane-oxidizing enzyme AlkB.,Guo X, Zhang J, Han L, Lee J, Williams SC, Forsberg A, Xu Y, Austin RN, Feng L Nat Commun. 2023 Apr 17;14(1):2180. doi: 10.1038/s41467-023-37869-z. PMID:37069165 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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